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Image Search Results
Journal: American Journal of Human Genetics
Article Title: Biallelic Mutations in NBAS Cause Recurrent Acute Liver Failure with Onset in Infancy
doi: 10.1016/j.ajhg.2015.05.009
Figure Lengend Snippet: Quantification of NBAS and p31 Protein Levels Mutant and control fibroblast cell lines were cultivated at 37°C. 10 μg protein of collected cells were separated on a 4%–12% acrylamide gradient gel, transferred to a PVDF membrane, and immunodecorated with antibodies against NBAS, p31, and β-actin. The antibody for NBAS was detecting a single protein band at approximately 270 kDa corresponding to the predicted molecular weight of NBAS. β-actin was used as a loading control. The quantified protein levels are based on three independent experiments and expressed as percentages of a control cell line, corrected for β-actin. Five of the eight investigated subject cell lines are shown. NBAS and p31 protein levels were severely reduced in fibroblast cell lines of RALF-affected individuals to 21% and 33% of control subjects, respectively. Error bar indicates 1 SD. Protein amount in control 1 was set as 100%.
Article Snippet: 10 μg of protein of every sample were separated on a 4%–12%
Techniques: Mutagenesis, Control, Membrane, Molecular Weight